强心苷类化合物对肺癌细胞中元素和化合物A549和H1975的不同生长抑制作用

来源：蜘蛛抓取(WebSpider) 时间：2017-11-03 10:32 标签： 细胞中的元素化合物
上传用户：finvaznofr资料价格：5财富值&&『』文档下载 ：『』&&『』学位专业：&关 键 词 ：&&&&&权力声明：若本站收录的文献无意侵犯了您的著作版权，请点击。摘要:（摘要内容经过系统自动伪原创处理以避免复制，下载原文正常，内容请直接查看目录。）目标结合运用表皮发展因子酪氨酸激酶克制剂一吉非替尼（Gefitinib）和放射线感化EGFR分歧状况的NSCLC细胞A549、H1975，不雅察该药对两种细胞放射敏理性、DSB修复情形的影响及其EGFR能否均在X线照耀后入核办法1)克隆构成试验检测各组细胞α、β和SF2等值；2)免疫荧鲜明示放疗后分歧时光点各组细胞核中磷酸化γ一H2AX的表达，剖析DSB修复情形；3)免疫印记检测各组细胞分歧时光点细胞核表里EGFR表达程度。成果1)加药组A549细胞SF2值为0。355低于对比组0。4443；加药组H1975细胞和对比组SF2值分离为0。07，无显著差别；2) A549细胞加药组比对比组各时光点γ一H2AX表达显著增高(T表现加药组均数±尺度差，t表现对比组均数±尺度差；T30min=22。33±2。30，t30min=14。18±2。61；T45min=15。95±2。40，t45min=11。40±1。75；T60min=11。47±1。19，t60min=4。40±0。58； P《0。05)；H1975细胞则无差别( T30min=22。07±0。56，t30min=21。59±1。52；T45min=15。97±0。90，t45min=15。63±0。93；T60min=11。39±0。39，t60min=10。83±1。29； P 》0。05)；3)加药组A549细胞比未加药组胞浆EGFR表达显著增长( T0min=18。1±0。306，t0min=12。3±0。557；T15min=18。2±0。306，t15min=11。1±0。379；T30min=16。2±0。551，t30min=7。37±0。451；T60min=15。5±0。265，t60min=4。53±0。379； P 《0。05)，胞核内EGFR明显削减( T15min=0。51±0。050，t15min=4。93±0。321；T30min=1。03±0。115，t30min=5。20±0。361；T60min=0。81±0。025，t60min=5。70±0。458； P 《0。05)；加药组H1975细胞和未加药组细胞照耀后胞核内没有EGFR表达，且胞浆内EGFR表达程度两组细胞无显著差异(T0min=1。80±0。042，t0min=1。73±0。07；T15min=1。76±0。043，t15min=1。51±0。040；T30min=1。68±0。040，t30min=1。75±0。051；T60min=1。70±0。021，t60min=1。65±0。036； P 》0。05)。结论吉非替尼能增长非小细胞肺癌A549放射敏理性，能够与吉非替尼克制A549放射线毁伤后的DSB修复，障碍EGFR入核有关；而对H1975细胞没有影响，能够与其放射后EGFR不入核相干。Abstract:Target binding by epidermal growth factor tyrosine kinase restraint agent a Ji non for Nepal (gefitinib) and ray effect of epidermal growth factor receptor (EGFR) differences in NSCLC cell line A549, and h1975, indecent observes the drug on two cell radiation sensitivity, DSB repair situation the influence and the EGFR can were in X-ray shine after nuclear way 1) clone composition test to detect the cell alpha, beta and SF2 2) immunofluorescence fresh express expression of H2AX phosphorylation of gamma in different time points in each group of the nucleus after radiotherapy, DSB repai 3) immune mark detected cell divides time nuclear cells inside and outside EGFR expression level. Results 1) dosing group A549 cells SF2 0. 355 lower than the contrast group of 0. 4443; dosing group H1975 cells and comparison group SF2 value is separated into 0. 3094 and 0. 3207, no si 2) A549 cells dosing group than contrast group each time point gamma a H2AX expression increased significantly (t performance dosing group were number + scale difference and t contrast group were number + T30min=22. 33 + 2. 30, t30min=14. 18 + 2. 61; T45min=15. 95 + 2. 40, t45min=11. 40 + 1. 75; T60min=11. 47 + 1. 19, t60min=4. 40 + 0. 58 P 0 &. 05); H1975 cells had no difference (T30min=22. 07 + 0. 56, t30min=21. 59 + 1. 52; T45min=15. 97 + 0. 90, t45min=15. 63 + 0. 93; T60min=11. 39 + 0. 39, t60min=10. 83 + 1. 29 P 0 &. 05); 3) dosing group A549 cells than no drug group of cytoplasmic EGFR expression was significantly increased (T0min=18. 1 + 0. 306, t0min=12. 3 + 0. 557; T15min=18. 2 + 0. 306, t15min=11. 1 + 0. 379; T30min=16. 2 + 0. 551, t30min=7. 37 + 0. 451; T60min=15. 5 + 0. 265, t60min=4. 53 + 0. 379 P 0 &. 05), in the nucleus of EGFR reduced significantly (T15min=0. 51 + 0. 050, t15min=4. 93 + 0. 321; T30min=1. 03 + 0. 115, t30min=5. 20 + 0. 361; T60min=0. 81 + 0. 025, t60min=5. 70 + 0. 458 P 0 &. 05); dosing group h1975 cell and no drug group cells shine after cell nuclear no expression of EGFR, and EGFR in cytoplasm expression degree of two groups were no significant difference (T0min=1. 80 + 0. 042, t0min=1. 73 + 0. 07; T15min=1. 76 + 0. 043, t15min=1. 51 + 0. 040; T30min=1. 68 + 0. 040, t30min=1. 75 + 0. 051; T60min=1. 70 + 0. 021, t60min=1. 65 + 0. 036 P 0 &. 05). Conclusion gefitinib imatinib can growth in non small cell lung cancer A549 radiation sensitivity, and gefitinib for nick of A549 radiation damage after DSB repair, obstacles EGFR
but have no effect on h1975 cell to and radiation after EGFR nuclear coherent.目录:中文摘要5-7英文摘要7-8前言9-10材料和方法10-17结果17-23讨论23-26参考文献26-28综述28-44&&&&参考文献39-44已发表文章44-46致谢46分享到：相关文献|扫扫二维码，随身浏览文档
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吉非替尼对肺癌细胞A549、H1975放射敏感性的影响及机制研究
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