产品名称：重组抗PD-1全人单
产品规格：500ug/1mg
包装说明：医用西林瓶
价格说明：750/1500元
产品数量：1000支
发布日期：
公司名称：
经营模式：生产型
联系方式：
留言询价：
&重组抗PD-1全人单克隆抗体(细胞培养级- PD1抗体，DC-CIk细胞治疗用PD1抗体&的 详 细 说 明
重组抗PD-1全人单克隆抗体(细胞培养级别)
PD1 全人单抗可以有效地封闭PDL1 和PD1 的结合，并且不会引起 HAMA 反应（人抗鼠抗体反应）。PD1 的抗体已作为广谱性抗肿瘤药物被接受，也可能成为最有效地平衡细胞治疗的工具。PD1 是激活的T细胞、B 细胞以及髓样细胞膜表面重要的免疫调控受体。与配体PDL1和PDL2 结合，抑制T 细胞增殖和细胞因子分泌，影响细胞治疗的效果。近来研究发现，DC 细胞含有PDL1，DC\CIK 联合治疗时，PDL1 可能是潜在的细胞治疗的负调节因素。PD\1 主要在活化的T、B 和NK 等细胞上呈诱导性表达,PD\1 有PD\L1(B7\H1,CD274)和PD\L2(B7\DC,CD273)两个配体。PD\L1 广泛组成性表达于多种实质器官组织、免疫细胞以及多种类型的肿瘤细胞上,而PD\L2 仅表达于活化的巨噬细胞、树突状细胞、骨髓来源的基质细胞和个别肿瘤细胞株。PD\1 随T 细胞活化程度逐步上调表达,与PD\L 结合后引发抑制信号的产生，致使效应性T 细胞失能并及时进入凋亡。 本产品系由单克隆细胞株表达并高度纯化后的抗体经超滤换液分装制成。 本产品为无菌澄明液体，由含有 10mM PBS pH为 7.2的蛋白溶液经0.2um过滤后分装。 &
货号：kx10-1& &&&&体积：50ul/500ul/1ml &&&&浓度：1mg/ml. &
纯& 度：经高效液相色谱（SEC-HPLC）和SDS-PAGE检测，纯度大于98.0%. 内毒素：小于1EU/mg. &
建议长期-80℃分装保存，无菌条件下操作，避免污染。 具体用量需通过预实验确定。
本公司还供应上述产品的同类产品：抗人CD3单克隆抗体,抗人CD28抗体,IL-4
北京科昕生物科技有限公司位于中关村科技园，专注于生命科学领域产品的研发，生产和销售。拥有GMP标准的中试车间和严格的产品质量控制体系，可以为客户提供细胞培养级别的重组蛋白和相关抗体。
科昕生物技术团队成员拥有丰富的蛋白质研发与生产经验,已建成包括大肠杆菌、酵母和哺乳动物细胞表达系统及相关的蛋白分离纯化平台，研发出多种重组蛋白质生产工艺,其质量标准能达到《2010版中国药典》第三部要求.
科昕生物将努力为中国的生物医药产业贡献一份自己的力量。科昕生物的诚信、实力和产品质量值得您信赖。
免责声明：以上所展示的信息由企业自行提供，内容的真实性、准确性和合法性由发布企业负责。新品快播网对此不承担任何保证责任。
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&行业目录导航：请问有没有人会看封闭抗体检查结果？？？ - 丫丫网 准备怀孕版
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热门推荐：
#1&&|&& 08:47
来自: 湖北 十堰)
ＣＤ３+细胞-2　　　　　86.6％参考是60--85（这个高一点）。&ＣＤ４+细胞　　 & & & & & &29.1％参考是24.5--48.8&ＣＤ８+细胞　　　　　 &33.5％参考18.5--42.1&ＣＤ４+细胞-２　　　 &28.2％参考24.5--48.8&ＣＤ８+细胞-２　　　 &32.6％参考18.5--42.1&TLB T淋巴细胞 & & & & & & 87.1参考值60--85%的（这个也高一点点）。&ＣＤ３-ＢＥ　　　　0.5&ＣＤ４-ＢＥ　　　　0.9&&ＣＤ８-ＢＥ　　　　0.9 后面三项参考大于0.0&整体看都是两项高于参考值。问了医生跑去市里省里，医生都说没问题，封闭抗体没问题。不知道会不会看，说没问题，我都三次胚育了都是没得原始心管搏动。求有经验的姐妹赐教。为什么我的检查结果不是直接显示阴阳性？这结果到底是正常吗？日 08:48, 等待等待 作第 2 次编辑
#2&&|&& 09:56
来自: 浙江 嘉兴)
封闭抗体系90%女性都是阴性，有些人怀孕后自己转阳，封闭抗体不是怀孕的绝对因素，如果有过流产停胎的人可以试着打封闭抗体针，打这针不是就能怀上，只是一个排除，一个疗程四针，这针很痛，怀上后前三个月要每月打一次拱固，特别是有过流产的人怀上后一定要打，希望能帮上忙。
#3&&|&& 19:36
来自: 河北 承德)
妹妹，我感觉你的怀孕过程太痛苦了，没有抓住重点。你听我的话，去买好艾条和艾灸盒，每天艾灸腹部正反面。艾条千万注意，大多数都是假艾条，这个不能开玩笑，千万选真艾条。所有的所谓金艾条都是假的，不要买。粉末状的艾条不要，艾绒是带点青色，卷儿的。中医的关键在药材正宗。艾草是纯阳的，可以把腹部的病，全部清理。如果哪天上火失眠，就用生地泡水喝，其他时间不需要药物和手术宫暖则孕，艾灸暖子宫，两个月以后，你再怀孕，不会再流产胎停了。你在***上搜索一下&艾灸任脉&，随便找个店铺，选两个七连罐的套装，绑在腹部正反面艾灸，每天一次，连续两个月。你去看单桂敏老师的博客，有怀孕篇。她家的艾条正宗与否我不清楚，不作推荐。
#4&&|&& 19:39
来自: 河北 承德)
胎停一般是宫内太寒。你说的子宫肌瘤问题，就说明里面寒，因为所有的肿瘤都是寒邪凝固。
#5&&|&& 13:06
来自: 上海 宝山)
道济 在第3楼写到妹妹，我感觉你的怀孕过程太痛苦了，没有抓住重点。你听我的话，去买好艾条和艾灸盒，每天艾灸腹部正反面。艾条千万注意，大多数都是假艾条，这个不能开玩笑，千万选真艾条。所有的所谓金艾条都是假的，不要买。粉末状的艾条不要，艾绒是带点青色，卷儿的。中医的关键在药材正宗。艾草是纯阳的，可以把腹部的病，全部清理。如果哪天上火失眠，就用生地泡水喝，其他时间不需要药物和手术宫暖则孕，艾灸暖子宫，两个月以后，你再怀孕，不会再流产胎停了。你在***上搜索一下“艾灸任脉”，随便找个店铺，选两个七连罐的套装，绑在腹部正反面艾灸，每天一次，连续两个月。你去看单桂敏老师的博客，有怀孕篇。她家的艾条正宗与否我不清楚，不作推荐。我是月经量很少，是少的只要迷你的那种或是护垫用用就可以 不知这个艾针炙可以改善吗，听说量少和宫寒也是有关系的
#6&&|&& 16:56
来自: 广东 广州)
一定会多多关注~~楼主
#7&&|&& 19:30
来自: 吉林 长春)
可以正常有排卵，亦能正常受精，但由于受精卵着床部位的子宫内膜发育不健全，以致影响受孕。这主要还是卵泡发育的事，对症用原治纯品的易孕胶囊，可以促排，营养卵泡使卵泡健康发育，从而提高成功受孕机率。
您还没有登录丫丫网，请先或者。Anti-PD1 antibody [NAT105] (ab52587) | Abcam
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Anti-PD1 antibody [NAT105] (ab52587)
Product nameAnti-PD1 antibody [NAT105]
DescriptionMouse monoclonal [NAT105] to PD1
Tested applicationsSuitable for:
WB, Flow Cyt, IHC-Fr, ICC/IF, IP, IHC-P
Species reactivityReacts with:
ImmunogenTissue, cells or virus corresponding to Human PD1. YT cells (human T/NK cell Leukemia)Database link:
General notesAlternative versions available: [NAT105] ()
Properties
FormLiquid
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS
PurityImmunogen affinity purified
ClonalityMonoclonal
Clone numberNAT105
IsotypeIgG1
Light chain typekappa
Research areas
Associated products
Compatible Secondaries
Applications
covers the use of
in the following tested applications.
The application notes include recommende optimal dilutions/concentrations should be determined by the end user.
Application
1/50. Predicted molecular weight: 32 kDa.
Use at an assay dependent concentration.
-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
1/200 - 1/1000.
Use at an assay dependent concentration.
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
FunctionPossible cell death inducer, in association with other factors.
Involvement in diseaseGenetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
Developmental stageInduced at programmed cell death.
Cellular localizationMembrane.
Database links
Alternative names
CD279 antibody
CD279 antigen antibody
hPD 1 antibody
Anti-PD1 antibody [NAT105] images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PD1 antibody [NAT] (ab52587)
Paraffin-embedded human tonsil section stained with ab52587 PD1 antibody at 1/50 dilution.
Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (ab52587)This image is courtesy of an anonymous Abreview
Immunohistochemical analysis of frozen Human liver tissue labeling PD1 with ab52587 at 1/50 dilution.
Immunohistochemistry (Paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
Double immunofluorescence staining of CD3 (green) and PD1 (red) on paraffin embedded tonsil.
Western blot - Anti-PD1 antibody [NAT105] (ab52587)This image is courtesy of an anonymous collaborator.
Anti-PD1 antibody [NAT105] (ab52587) at 1/50 dilution + YT cell line extractsPredicted band size : 32 kDaObserved band size : 47 kDa ()This image is courtesy of an anonymous collaborator.
Immunohistochemistry (Paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)
Double immunoenzymatic staining of Ki67 (brown) and PD1 (red) on paraffin embedded tonsil.
Flow Cytometry - Anti-PD1 antibody [NAT] (ab52587)
Sample: Human tonsil cell extract
Dilution: ab52587 antibody was used as 1:200 in 1x106 cells/tube.
Anti-CD4 antibody was used as 1:200 dilution
Anti-CD8 antibody was used as 1:200 dilution
Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT] (ab52587)
Tissue Sample: Human Tonsil ab52587 diluted 1:100Incubation time: 30 mins
References for Anti-PD1 antibody [NAT105] (ab52587)
This product has been referenced in:
Bastman JJ &et al. Tumor-infiltrating T Cells and the PD-1 Checkpoint Pathway in Advanced Differentiated and Anaplastic Thyroid Cancer. J Clin Endocrinol Metab N/A:jc16).
Joseph RW &et al. Lichenoid dermatitis in three patients with metastatic melanoma treated with anti-PD-1 therapy. Cancer Immunol Res 3:18-22 (2015).
Find concentration of your lot:
13 Abreviews or Q&A
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used:
Monkey Tissue sections (Lymphoid tisssues - spleen, tonsil and lymph node)
Specification
Lymphoid tisssues - spleen, tonsil and lymph node
Permeabilization
Formaldehyde
Abcam user community
Verified customer
this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Human Tissue sections (Liver)
Specification
Acetone/Methanol 1:2
Permeabilization
Yes - TBS + 0,1% TritonX100
Blocking step
Serum as blocking agent for 10 minute(s) & Concentration: 10% & Temperature: 20&C
Abcam user community
Verified customer
this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Follicular Lymphoma, Lymph node)
Specification
Follicular Lymphoma, Lymph node
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used:
Citrate Buffer, pH6
Blocking step
Novolink Protein Block, Novocastra, Leica Microsystems Inc. as blocking agent for 10 minute(s) & Concentration: 100% & Temperature: 25&C
Dr. Hajnalka Rajnai
Verified customer
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Human lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used:
Low pH buffer
Permeabilization
Specification
Human lymph node
Blocking step
Dako endogenouse peroxidase block as blocking agent for 5 minute(s) & Concentration: 100% & Temperature: RT&C
Paraformaldehyde
Abcam user community
Verified customer
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Prostate Cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used:
Citrate buffer pH 6.0
Permeabilization
Specification
Prostate Cancer
Blocking step
Serum as blocking agent for 15 minute(s) & Concentration: 10% & Temperature: 23&C
Formaldehyde
Abcam user community
Verified customer
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako Flex peroxidase as blocking agent for 5 minute(s) & Concentration: 100% & Temperature: RT&C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used:
Human Tissue sections (Malignant Melanoma)
Specification
Malignant Melanoma
Permeabilization
Formaldehyde
Abcam user community
Verified customer
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used:
TRIS-EDTA Buffer ph 8,5
Human Tissue sections (tonsil)
Specification
Paraformaldehyde
Mr. Rudolf Jung
Verified customer
Abcam community
Verified customer
.You may want to consider the following secondary antibodies:/donkey-fab2-polyclonal-secondary-antibody-to-mouse-igg-hl-alexa-fluorreg-488-pre-adsorbed-ab150101.html/donkey-fab2-poly...
Abcam Scientific Support
Abcam community
Verified customer
I can confirm that the epitope of ab52587 is extracellular, because in FACS experiments fresh, unfixed cells were used.
Abcam Scientific Support
Abcam community
Verified customer
I can suggest the following secondary antibodies:
goat anti-mouse antibody
/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98808.html
the rabbit anti-mouse antibody
http://www....
Abcam Scientific Support
Please note: All products are &FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE&百度知道 - 信息提示
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